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Primer Design for ClonExpress Entry
Primer Design for ClonExpress EntrySingle-Fragment Cloning
Single-Fragment Cloning (ClonExpress II)Multi-Fragment Assembly
Multi-Fragment Assembly (ClonExpress MultiS)Single-Point Mutation
Single-Point Mutation (MutExpress II)Double/Multi-Point Mutations
Double/Multi-Point Mutations (MutExpress II / MultiS)Calculator of Input DNA Amounts
Calculator of Input DNA AmountsDNA Sequence Processing Tools
DNA Sequence Processing ToolsTm Calculator
Tm Calculator
Click to view the instructions
This software can be used to calculate the optimal DNA input amount for the recombinant reaction.
1. First, select the experiment type from the dropdown menu, such as "Entry Cloning" or "Seamless Cloning." If "Seamless Cloning" is selected, please proceed to select the number of inserts.
2. In the corresponding text box below, enter the DNA fragment length information (unit must be in kb). The input amount for the recombinant reaction (unit: ng) will be displayed instantly in the label on the right.
* Select the reaction type:
* Please enter the length of insert : kb
Recommended amount for recombination reaction:
ng
* Please enter the length of vector : kb
Recommended amount for recombination reaction:
* Please enter the length of insert : kb
Recommended amount for recombination reaction:
* Select the number of sites to be mutated:
* Please enter the length of vector: kb * Please enter the concentration of the cloning vector: ng/μl
The optimal input amount:
ng
The volume:
μl
* Please enter the length of insert 1 : kb * Please enter the concentration of the insert 1 : ng/μl
The optimal input amount:
ng
The volume:
μl
* Please enter the length of insert 2 : kb * Please enter the concentration of the insert 2 : ng/μl
The optimal input amount:
ng
The volume:
μl
* Please enter the length of insert 3 : kb * Please enter the concentration of the insert 3 : ng/μl
The optimal input amount:
ng
The volume:
μl
* Please enter the length of insert 4 : kb * Please enter the concentration of the insert 4 : ng/μl
The optimal input amount:
ng
The volume:
μl
* Please enter the length of insert 5 : kb * Please enter the concentration of the insert 5 : ng/μl
The optimal input amount:
ng
The volume:
μl
* Please enter the length of insert 6 : kb * Please enter the concentration of the insert 6 : ng/μl
The optimal input amount:
ng
The volume:
μl
* Please enter the length of insert 7 : kb * Please enter the concentration of the insert 7 : ng/μl
The optimal input amount:
ng
The volume:
μl
* Please enter the length of insert 8 : kb * Please enter the concentration of the insert 8 : ng/μl
The optimal input amount:
ng
The volume:
μl
* Please enter the length of insert 9 : kb * Please enter the concentration of the insert 9 : ng/μl
The optimal input amount:
ng
The volume:
μl
* Please enter the length of insert 10 : kb * Please enter the concentration of the insert 10 : ng/μl
The optimal input amount:
ng
The volume:
μl
* Please enter the length of insert 11 : kb * Please enter the concentration of the insert 11 : ng/μl
The optimal input amount:
ng
The volume:
μl
Note:
Note:
* Please enter the length of vector to be mutated : kb
Recommended amount for recombination reaction:
* Please enter the longer DNA length between the two mutation sites: kb
Recommended amount for recombination reaction:
* Please enter the shorter DNA length between the two mutation sites: kb
Recommended amount for recombination reaction:
* Select the number of sites to be mutated:
* Please enter the DNA length between mutation site 1 and mutation site 2: kb
Recommended amount for recombination reaction:
* Please enter the DNA length between mutation site 2 and mutation site 3: kb
Recommended amount for recombination reaction:
* Please enter the DNA length between mutation site 3 and mutation site 1: kb
Recommended amount for recombination reaction:
* Please enter the DNA length between mutation site 3 and mutation site 4: kb
Recommended amount for recombination reaction:
* Please enter the DNA length between mutation site 4 and mutation site 1: kb
Recommended amount for recombination reaction:
* Please enter the DNA length between mutation site 4 and mutation site 5: kb
Recommended amount for recombination reaction:
* Please enter the DNA length between mutation site 5 and mutation site 1: kb
Recommended amount for recombination reaction: